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Biological Kits Information
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| Product Name |
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NFkappaB p50 Transcription Factor Assay kit |
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| Catalog Number |
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70-515 |
| Components |
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Transcription Factor Assay Plate (Part No. 90268): One 96-well Streptavidin coated plate sealed in a foil pouch with desiccant,TFA Buffer, 5x (Part No. 90272): One bottle containing 80 mL of 5x Transcription Factor Assay Buffer containing sonicated salmon sperm DNA to block non-specific DNA binding activity. |
| Unit Size |
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1 plate |
| Description |
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The Non-Radioactive NFkB p50 Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the consensus sequence for NFkB binding (5'-GGGACTTTCC-3'), is mixed with cellular (nuclear) extract in the Transcription Factor Assay Buffer provided. When incubated together, the active form of NFkB contained in the nuclear extract binds to its consensus sequence. After incubation, the extract/probe/buffer mixture is then directly transferred to the streptavidin coated plate. The biotinylated double stranded oligonucleotide bound by active NFkB protein is immobilized and any inactive, unbound material is washed away. The bound NFkB transcription factor subunit, p50, is detected with a specific primary antibody, a Rabbit anti-NFkB p50 (active form). An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader. Included in the kit are positive cell extract, a non-specific double stranded oligonucleotide, and a specific competitor double stranded oligonucleotide. |
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| Method |
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The transcription factor NFkB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFkB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFkB1 (p50 and its precursor p105), and NFkB2 (p52 and its precursor p100). NF-kB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFkB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFkB is the most abundant in cells. |
| Storage |
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20°C |
| Request Info |
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| Supplier |
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Upstate |
| Contact Info |
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28820 Single Oak Drive
Temecula, CA 92590
United States.
Phone:781 890 8845 |
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